Restriction Site Map Generator
Enter a DNA sequence in the box below to analyze the sequence for restriction sites and generate a restriction map.
Want a better tool to analyze DNA and Protein sequences? Try our free program GeneCoder
The following restriction enzymes are available with this tool:
AatII (gacgtc) | AccI (gtmkac) | AflII (cttaag) | AflIII (acrygt) |
AhaII (grcgyc) | AluI (AGCT) | AlwI (ggatc) | AlwNI (cagnnnctg) |
ApaI (gggccc) | ApaLI (gtgcac) | AseI (attaat) | Asp718 (ggtacc) |
AvaI (cycgrg) | AvaII (ggwcc) | AvrII (cctagg) | BamHI (ggatcc) |
BanI (ggyrcc) | BanII (grgcyc) | BbeI (ggcgcc) | BbvI (gcagc) |
BbvII (gaagac) | BclI (tgatca) | BcnI (ccsgg) | BglI (gccn5ggc) |
BglII (agatct) | BsaAI (yacgtr) | BsmAI (gtctc) | Bsp1286I (gdgchc) |
BspHI (tcatga) | BspMI (acctgc) | BspMII (tccgga) | BssHII (gcgcgc) |
BstBI (ttcgaa) | BstEII (ggtnacc) | BstNI (ccwgg) | BstUI (cgcg) |
BstXI (ccan6tgg) | BstYI (rgatcy) | Bsu36I (cctnagg) | Cfr10I (rccggy) |
ClaI (atcgat) | DdeI (ctnag) | DpnI (gatc) | DraI (tttaaa) |
DraIII (cacnnngtg) | DrdI (gacn6gtc) | DsaI (ccrygg) | EaeI (yggccr) |
EagI (cggccg) | EarI (ctcttc) | Eco47III (agcgct) | Eco57I (ctgaag) |
EcoNI (cctn5agg) | Eco0109I (rggnccy) | EcoRI (gaattc) | EcoRII (ccwgg) |
EcoRV (gatatc) | EspI (gctnagc) | Fnu4HI (gcngc) | FokI (ggatg) |
FspI (tgcgca) | GsuI (ctggag) | HaeI (wggccw) | HaeII (rgcgcy) |
HaeIII (ggcc) | HgaI (gacgc) | HgiAI (gwgcwc) | HgiEII (accn6ggt) |
HhaI (gcgc) | HinCII (gtyrac) | HindIII (aagctt) | HinfI (gantc) |
HinPI (gcgc) | HpaI (gttaac) | HpaII (ccgg) | HphI (ggtga) |
KpnI (ggtacc) | MaeI (ctag) | MaeII (acgt) | MaeIII (gtnac) |
MboI (gatc) | MboII (gaaga) | MluI (acgcgt) | MmeI (tccrac) |
MnlI (cctc) | MscI (tggcca) | MseI (ttaa) | MspI (ccgg) |
NaeI (gccggc) | NarI (ggcgcc) | NciI (ccsgg) | NcoI (ccatgg) |
NdeI (catatg) | NheI (gctagc) | NlaIII (catg) | NlaIV (ggnncc) |
NotI (gcggccgc) | NruI (tcgcga) | NsiI (atgcat) | Nsp7524I (rcatgy) |
NspBII (cmgckg) | NspHI (rcatgy) | PaeR7I (ctcgag) | PflMI (ccan5tgg) |
PleI (gagtc) | PmlI (cacgtg) | PpuMI (rggwccy) | PstI (ctgcag) |
PvuI (cgatcg) | PvuII (cagctg) | RsaI (gtac) | RsrII (cggwccg) |
SacI (gagctc) | SacII (ccgcgg) | SalI (gtcgac) | Sau3AI (gatc) |
Sau96I (ggncc) | ScaI (agtact) | ScrFI (ccngg) | SecI (ccnngg) |
SfaNI (gcatc) | SfiI (ggccn5ggcc) | SmaI (cccggg) | SnaBI (tacgta) |
SpeI (actagt) | SphI (gcatgc) | SplI (cgtacg) | SspI (aatatt) |
StuI (aggcct) | StyI (ccwwgg) | TaqI (tcga) | Tth111I (gacnnngtc) |
Tth111II (caarca) | XbaI (tctaga) | XcaI (gtatac) | XhoI (ctcgag) |
XcmI (ccan9tgg) | XmaI (cccggg) | XmnI (gaan4ttc) |